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shrna plasmid with pspax2  (Addgene inc)


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    Structured Review

    Addgene inc shrna plasmid with pspax2
    Shrna Plasmid With Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pspax2/psPAX2+(Plasmid+%2312260)/pmc13050104-51-9-13
    Average 98 stars, based on 15515 article reviews
    shrna plasmid with pspax2 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    other:

    Article Title: A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types.
    Article Snippet: Lentiviruses were packaged with plasmids psPAX2 and pMD2.G (Addgene) in HEK 293T cells.

    Article Title: CLN3 mediates chloride efflux from lysosomes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Fisher Scientific Cat# A452SK-4 2-propanol Sigma-Aldrich Cat# 1.02781 Triton-X 100 Sigma-Aldrich Cat# X100 isoflurane Dechra N/A DMEM Thermo Fisher Scientific Cat# 10566016 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5670701 TrypLE express enzyme (1X), no phenol red Thermo Fisher Scientific Cat# 12604039 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 MEM amino acids (50x) solution Sigma-Aldrich Cat# M5550 HBSS, no calcium, no magnesium, no phenol red Thermo Fisher Scientific Cat# 14175095 JetOPTIMUS DNA transfection reagent Polyplus Cat# 101000006 Polybrene Sigma-Aldrich Cat# H9268 Puromycin Sigma-Aldrich Cat# P8833 Poly-L-lysine hydrobromide Sigma Aldrich Cat# P1399 Vacuolin-1 Sigma Aldrich Cat# 673000 Nigericin (sodium salt) MedChemExpress (MCE) Cat# HY-100381 Valinomycin Sigma Aldrich Cat# 94675 Tributyltin chloride Sigma Aldrich Cat# T50202 Monensin sodium salt MedChemExpress (MCE) Cat# HY-N0150 Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat# D8418 DIDS Tocris Bioscience Cat# 4523 Niflumic acid Sigma Aldrich Cat# N0630 5-Nitro-2-(3phenylpropylamino)benzoic acid Sigma Aldrich Cat# N4779 Curcumin analog C1 SelleckChem Cat# S6769 Eltrombopag MedChemExpress (MCE) Cat# HY-15306 DTT Thermo Fisher Scientific Cat# P2325 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1600-100 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-121 Protease inhibitor cocktail Sigma Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail I Sigma Aldrich Cat# 4906845001 LDS sample buffer (4x) Thermo Fisher Scientific Cat# NP0008 Pierce BCA Protein Assay Thermo Fisher Scientific Cat# 23227 Intercept (TBS) Blocking Buffer LI-COR Cat# 927-60001 20xTBS Tween-20 Buffer Thermo Fisher Scientific Cat# 28360 PVDF membrane Sigma Aldrich Cat# IPFL00010 TRIzol Reagent Thermo Fisher Scientific Cat# 15596026 RIPA This paper N/A LysoSensor Green DND-189 Thermo Fisher Scientific Cat# L7535 Dextran, Oregon Green 488 Thermo Fisher Scientific Cat# D7170 Magic Red Cathepsin L Immunochemistry Cat# 941 DQ-BSA red dye Thermo Fisher Scientific Cat# D12051 Dil Thermo Fisher Scientific Cat# D282 Critical commercial assays Q5 Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S GoTaq G2 Green Master Mix Promega Cat# M7822 ClonExpress II One Step Cloning Kit Vazyme Cat# C112 2 × Phanta Flash Master Mix (Dye Plus) Vazyme Cat# P520 (Continued on next page) e2 Neuron 114, 1–16.e1–e7, March 4, 2026

    Article Title: Selective proteasomal degradation from the Golgi apparatus membrane
    Article Snippet: The plasmids psPAX2 and pMD2.G were gifts from Didier Trono (Addgene plasmids #12260 and #12259).

    Article Title: Targeting Prolyl 3-hydroxylase 1 inhibits pancreatic cancer progression and macrophage immunity.
    Article Snippet: Plasmids psPAX2 (#12260) and pMD2.G (#12259) were acquired from Addgene.

    Plasmid Preparation:

    Article Title: Regulation of sodium/calcium homeostasis by BacNa v gene therapy rescues cardiac dysfunction in chronic heart failure
    Article Snippet: .. Plasmids psPAX2 and pMD2.G were obtained from D. Trono (Addgene plasmid nos. ..

    Article Title: Regulation of sodium/calcium homeostasis by BacNa v gene therapy rescues cardiac dysfunction in chronic heart failure.
    Article Snippet: .. Plasmids psPAX2 and pMD2.G were obtained from D. Trono (Addgene plasmid nos. ..

    Article Title: ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus
    Article Snippet: The resulting PCR product ( ) was cloned by Gibson assembly into pLenti-Puro-AU-flip-3xBsmBI (Addgene #196709). .. The plasmid library was input sequenced to confirm representation and packaged into lentivirus in HEK293T (ATCC) using plasmids psPAX2 (a gift from Didier Trono, Addgene #12260) and pCMV-VSV-G (a gift from Bob Weinberg, Addgene #8454). .. The virus-containing supernatant was concentrated with Lenti-X concentrator (Takara, Saint-Germain-en-Laye, France), aliquoted, and stored in liquid nitrogen.

    Transfection:

    Article Title: The phosphate exporter XPR1 regulates a gasdermin D–independent mature IL-1β secretion pathway in LPS-stimulated human monocytic cells
    Article Snippet: The gRNA pooled library in lentiCRISPRv2 was amplified and sequenced according to the accompanying online manual ( https://www.addgene.org/pooled-library/broadgpp-human-knockout-brunello/ ). .. For production of the lentiviral pooled library, 60 × 10 6 HEK293T cells were transfected with the plasmids psPAX2 (Addgene, #12260) and pCMV-VSV-G (Addgene, #8454) and the Brunello library in LentiCRISPRv2, in 10-cm dishes at a confluence of 70% with TransIT-LT1 (Mirus, MIR-2304) according to the provider’s protocol. .. Viral supernatants were collected 48 h post-transfection and passed through a 0.45-μm PVDF filter via a syringe, and viruses were concentrated 25-fold using Vivaspin 20 centrifugal concentrators (Sigma-Aldrich, Z614637).



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